ABSTRACT
Background: Biotic and abiotic elicitors produce secondary metabolites and alter physiological traits of the plants by affecting some of the mechanisms within the plant cells
Objective: This study was aimed to investigate the effects of different concentrations of spherical nano-carbon and salicylic acid on physiological traits and paratolenolidae content in Feverfew
Methods: This research was conducted in a factorial experiment based on randomized completely design [CRD] under greenhouse conditions. The first factor was Feverfew cultivar with two levels [T.parthenium Linn. cv. Jelitto and T. parthenium Linn. cv. Pharmasaat], the second factor was foliar spray of spherical nano-carbon with 5 levels [0, 125, 250, 500 and 1000 mg/L] and the third factor was considered as foliar spray of salicylic acid with two levels [0 and 0.2 mM]
Results: The results showed that the cultivars, different concentrations of spherical nano-carbon, salicylic acid and their interactions had a significant effect on some of the studied traits. Two cultivars in the content of chlorophyll a and b and parthenolide showed significant differences at P<0.01. Also, the effect of spherical nano-carbon and salicylic acid concentrations on parethenolide content of extract was significant [P <0.01]. However, the highest content of parthenolide [24.22 Mug/0.1 g dry matter] was observed in pharmasaat and at the concentration of 500 mg/L spherical nano-carbon without salicylic acid application
Conclusion: Application of spherical nano-carbon at concentration of 500 mg/L along with 0.2 mM salicylic acid improved physiological traits and metabolic content of both cultivars
ABSTRACT
Satureja bachtiarica Bunge, an endemic species with relatively wide distribution, is traditionally used as a medicinal and spice plant in Iran. Essential oils composition, antioxidant activities and phenolics content of wild and cultivated S. bachtiarica of Yazd origin were determined in this study. Hydrodistilled essential oils were analyzed by GC-FID and GC-MS. Folin-Ciocalteu and A1C1[3] methods were served to determine total phenolics and flavonoids of methanol extracts, respectively. Antioxidant activities of the extracts were examined by DPPH and FRAP methods and their rosmarinic acid content was measured by using HPLC. The oil content of cultivated and wild plants was 1.8% and 0.9% v/w, respectively. Oxygenated monoterpens [78.3 - 79.5%] constituted the major fraction of essential oils with carvacrol [71.4% and 57.4% in cultivated and wild plants, respectively- Cymene [8.6 - 12.5%] was another major components of essential oils. Methanol extract from wild growing plants exhibited the higher levels antioxidant activities in both DPPH and FRAP methods [29.04 and 58.25 mg Trolox/g, respectively]. This extract also contained the higher amounts of total phenolics [24.5 mg caffeic acid /g sample], total flavonoids [87.99 mg catechin /g sample] and rosmarinic acid [3.73 mg/g dry weight] in comparison to cultivated ones [16.2 mg caffeic acid /g sample, 40.55 mg catechin /g sample and 1.73 mg/g dry weight, respectively]. Obtained results may be helpful for domestication of this plant and development of natural antioxidants for use in different industries